Overview

  • Product nameAnti-SLC47A1 antibody
    See all SLC47A1 primary antibodies
  • Description
    Rabbit polyclonal to SLC47A1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 500-530 of Human SLC47A1, conjugated to KLH.

  • Positive control
    • MD-MBA435 cell lysate IHC-P: FFPE human liver tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab104016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionSolute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes.
  • Tissue specificityExpressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney (especially found in luminal membranes of the urinary tubules, bile caniculi and brush border membranes).
  • Sequence similaritiesBelongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.
  • Cellular localizationCell membrane. Predominantly localized at the plasma membrane but also found in intracellular organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10847 antibody
    • hMATE-1 antibody
    • hMATE1 antibody
    • MATE-1 antibody
    • MATE1 antibody
    • MGC6482 antibody
    • multidrug and toxin extrusion 1 antibody
    • Multidrug and toxin extrusion protein 1 antibody
    • S47A1_HUMAN antibody
    • SLC47A1 antibody
    • Solute carrier family 47 member 1 antibody
    see all

Anti-SLC47A1 antibody images

  • IHC image of SLC47A1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104016, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-SLC47A1 antibody (ab104016) at 1/1000 dilution + MDA-MB-435 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 62 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

References for Anti-SLC47A1 antibody (ab104016)

This product has been referenced in:
  • Schmidt-Lauber C  et al. Transport mechanisms and their pathology-induced regulation govern tyrosine kinase inhibitor delivery in rheumatoid arthritis. PLoS One 7:e52247 (2012). WB ; Human . Read more (PubMed: 23284953) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"