Anti-MATE-1 antibody (ab92295)
Key features and details
- Goat polyclonal to MATE-1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MATE-1 antibody
See all MATE-1 primary antibodies -
Description
Goat polyclonal to MATE-1 -
Host species
Goat -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MATE-1 aa 528-541 (internal sequence) (Cysteine residue).
Sequence:C-PEHPQDGAKLSRKQ
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Positive control
- WB: Human liver tissue lysate; ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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ICC
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide (NMN), metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, ganciclovir and also the zwitterionic cephalosporin, cephalexin and cephradin. Seems to also play a role in the uptake of oxaliplatin (a new platinum anticancer agent). Able to transport paraquat (PQ or N,N-dimethyl-4-4'-bipiridinium); a widely used herbicid. Responsible for the secretion of cationic drugs across the brush border membranes. -
Tissue specificity
Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney (especially found in luminal membranes of the urinary tubules, bile caniculi and brush border membranes). -
Sequence similarities
Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. -
Cellular localization
Cell membrane. Predominantly localized at the plasma membrane but also found in intracellular organelles. - Information by UniProt
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Database links
- Entrez Gene: 55244 Human
- Omim: 609832 Human
- SwissProt: Q96FL8 Human
- Unigene: 232054 Human
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Alternative names
- FLJ10847 antibody
- hMATE-1 antibody
- hMATE1 antibody
see all
Images
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Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells labeling MATE-1. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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All lanes : Anti-MATE-1 antibody (ab92295) at 0.1 µg/ml
Lane 1 : Human liver lysate (in RIPA buffer)
Lane 2 : Human liver lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 62 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (0)
ab92295 has not yet been referenced specifically in any publications.