Overview

  • Product nameAnti-SLC47A2 antibody
    See all SLC47A2 primary antibodies
  • Description
    Rabbit polyclonal to SLC47A2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 506-555 (TYSRSECHVD FFRTPEEAHA LSAPTSRLSV KQLVIRRGAA LGAASATLMV) of Human SLC47A2 (NP_001093116).

  • Positive control
    • Human fetal lung lysate IHC-P: FFPE human kidney tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab106117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.Can be blocked with SLC47A2 peptide (ab156863). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSolute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide, metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, and ganciclovir. Responsible for the secretion of cationic drugs across the brush border membranes.
  • Tissue specificityIsoform 3 is predominantly expressed in kidney. Isoform 6 is expressed in brain.
  • Sequence similaritiesBelongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.
  • Cellular localizationCell membrane. Localized at the brush border membranes of the proximal tubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • H(+)/organic cation antiporter kidney-specific antibody
    • H+/organic cation antiporter antibody
    • hMATE-2 antibody
    • Kidney-specific H(+)/organic cation antiporter antibody
    • MATE-2 antibody
    • MATE2 B antibody
    • MATE2 K antibody
    • MATE2K antibody
    • Multidrug and toxin extrusion 2 antibody
    • Multidrug and toxin extrusion protein 2 antibody
    • S47A2_HUMAN antibody
    • SLC47A2 antibody
    • Solute carrier family 47 member 2 antibody
    see all

Anti-SLC47A2 antibody images

  • IHC image of SLC47A2 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106117, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-SLC47A2 antibody (ab106117) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size : 61 kDa
    Gel concentration: 12%

References for Anti-SLC47A2 antibody (ab106117)

ab106117 has not yet been referenced specifically in any publications.

Product Wall

The peptide is available as ab156863.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"