IHC image of SLC4A7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - SLC4A7 antibody (ab82335)
All lanes : Anti-SLC4A7 antibody (ab82335) at 1 µg/ml
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 136 kDa Observed band size : 136 kDa Additional bands at : 19 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
References for Anti-SLC4A7 antibody (ab82335)
This product has been referenced in:
Namkoong E et al. Role of Sodium Bicarbonate Cotransporters in Intracellular pH Regulation and Their Regulatory Mechanisms in Human Submandibular Glands. PLoS One10:e0138368 (2015).
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