Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC4A7
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human SLC4A7.

    (Peptide available as ab96634.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Caco2 and HEK293.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).

Target

  • Relevance
    SLC4A7 regulates intracellular pH and may also play a role in bicarbonate salvage in secretory epithelia. Additionally, SLC4A7 may also have associated sodium channel activity.
  • Cellular localization
    Basolateral cell membrane; Multi pass membrane protein. Apical cell membrane; Multi pass membrane protein. Cell projection, stereocilium.
  • Database links
  • Alternative names
    • Bicarbonate transporter antibody
    • BT antibody
    • NBC2 antibody
    • NBC3 antibody
    • S4A7 antibody
    • SBC2 antibody
    • SLC4A6 antibody
    • Sodium bicarbonate cotransporter 2 antibody
    • Sodium bicarbonate cotransporter 2b antibody
    • Sodium bicarbonate cotransporter 3 antibody
    • Solute carrier family 4 member 7 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 7 antibody
    see all

Anti-SLC4A7 antibody images

  • IHC image of SLC4A7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-SLC4A7 antibody (ab82335) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 136 kDa
    Observed band size : 136 kDa
    Additional bands at : 19 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-SLC4A7 antibody (ab82335)

This product has been referenced in:
  • Namkoong E  et al. Role of Sodium Bicarbonate Cotransporters in Intracellular pH Regulation and Their Regulatory Mechanisms in Human Submandibular Glands. PLoS One 10:e0138368 (2015). Human . Read more (PubMed: 26375462) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab82335.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up