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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).
Relevance SLC4A7 regulates intracellular pH and may also play a role in bicarbonate salvage in secretory epithelia. Additionally, SLC4A7 may also have associated sodium channel activity.
Cellular localization Basolateral cell membrane; Multi pass membrane protein. Apical cell membrane; Multi pass membrane protein. Cell projection, stereocilium.
Bicarbonate transporter antibody
Anti-SLC4A7 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SLC4A7 antibody (ab82335)
IHC image of SLC4A7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - SLC4A7 antibody (ab82335)
All lanes : Anti-SLC4A7 antibody (ab82335) at 1 µg/ml Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg developed using the ECL technique Performed under reducing conditions. Predicted band size : 136 kDa Observed band size : 136 kDa Additional bands at : 19 kDa,60 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
References for Anti-SLC4A7 antibody (ab82335)
has not yet been referenced specifically in any publications.
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