• Product name
  • Description
    Rabbit polyclonal to SLC4A7
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human SLC4A7.

    (Peptide available as ab96634.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Caco2 and HEK293.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab82335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Relevance
    SLC4A7 regulates intracellular pH and may also play a role in bicarbonate salvage in secretory epithelia. Additionally, SLC4A7 may also have associated sodium channel activity.
  • Cellular localization
    Basolateral cell membrane; Multi pass membrane protein. Apical cell membrane; Multi pass membrane protein. Cell projection, stereocilium.
  • Database links
  • Alternative names
    • Bicarbonate transporter antibody
    • BT antibody
    • NBC2 antibody
    • NBC3 antibody
    • S4A7 antibody
    • SBC2 antibody
    • SLC4A6 antibody
    • Sodium bicarbonate cotransporter 2 antibody
    • Sodium bicarbonate cotransporter 2b antibody
    • Sodium bicarbonate cotransporter 3 antibody
    • Solute carrier family 4 member 7 antibody
    • Solute carrier family 4 sodium bicarbonate cotransporter member 7 antibody
    see all


  • IHC image of SLC4A7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82335, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-SLC4A7 antibody (ab82335) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 136 kDa
    Observed band size: 136 kDa
    Additional bands at: 19 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds


This product has been referenced in:
  • Xie ZD  et al. The Balance of [Formula: see text] Secretion vs. Reabsorption in the Endometrial Epithelium Regulates Uterine Fluid pH. Front Physiol 9:12 (2018). WB, IHC-Fr . Read more (PubMed: 29422866) »
  • Namkoong E  et al. Role of Sodium Bicarbonate Cotransporters in Intracellular pH Regulation and Their Regulatory Mechanisms in Human Submandibular Glands. PLoS One 10:e0138368 (2015). Human . Read more (PubMed: 26375462) »

See all 2 Publications for this product

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