Overview

  • Product nameAnti-SLC6A15 antibody
    See all SLC6A15 primary antibodies
  • Description
    Rabbit polyclonal to SLC6A15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 180-229 (DQCPLVKNAS HTFVEPECEQ SSATTYYWYR EALNISSSIS ESGGLNWKMT) of Human SLC6A15 (NP_877499).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionFunctions as a sodium-dependent neutral amino acid transporter. Exhibits preference for the branched-chain amino acids, particulary leucine, valine and isoleucine and methionine. Mediates the saturable, pH-sensitive and electrogenic cotransport of proline and sodium ions with a stoichiometry of 1:1. May have a role as transporter for neurotransmitter precursors into neurons. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent.
  • Tissue specificityAlmost exclusively expressed in the brain.
  • Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A15 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • hv7 3 antibody
    • MGC87066 antibody
    • NTT73 antibody
    • Orphan sodium and chloride dependent neurotransmitter transporter NTT73 antibody
    • Orphan transporter v7 3 antibody
    • S6A15_HUMAN antibody
    • SBAT1 antibody
    • SLC6A15 antibody
    • sodium- and chloride-dependent neurotransmitter transporter NTT73 antibody
    • Sodium-coupled branched-chain amino-acid transporter 1 antibody
    • Sodium-dependent neutral amino acid transporter B(0)AT2 antibody
    • Solute carrier family 6 (neutral amino acid transporter) member 15 antibody
    • Solute carrier family 6 member 15 antibody
    • Transporter v7-3 antibody
    • V7 3 antibody
    see all

Anti-SLC6A15 antibody images

  • Anti-SLC6A15 antibody (ab84621) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SLC6A15 antibody (ab84621)

ab84621 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brian hippocampal tissue)
Loading amount 40 µg
Specification Brian hippocampal tissue
Gel Running Conditions Non-reduced Denaturing (10% (Bis-Tris))
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"