Overview

  • Product nameAnti-SLC6A7 antibody
    See all SLC6A7 primary antibodies
  • Description
    Rabbit polyclonal to SLC6A7
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SLTSDLPWEH CGNWWNTELC LEHRVSKDGN GALPLNLTCT VSPSEEYWSR YVLHIQGSQG IGSPG, corresponding to amino acids 147-211 of Human SLC6A7

  • Positive control
    • Human colon tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTerminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Tissue specificityBrain.
  • Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A7 subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • solute carrier family 6 (neurotransmitter transporter, L-proline), member 7 antibody
    • brain-specific L-proline transporter antibody
    • L-proline transporter, brain-specific antibody
    • PROT antibody
    • SC6A7_HUMAN antibody
    • Slc6a7 antibody
    • Sodium-dependent proline transporter antibody
    • solute carrier family 6 (neurotransmitter transporter), member 7 antibody
    • Solute carrier family 6 member 7 antibody
    see all

Anti-SLC6A7 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab122024 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122024, at a 1/20 dilution, staining SLC6A7 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

References for Anti-SLC6A7 antibody (ab122024)

ab122024 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Human Cultured Cells (CHO)
Specification CHO
Fixative Formaldehyde
Permeabilization Yes - 0.3% triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted May 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"