The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 71, <36 kDa (predicted molecular weight: 71 kDa).Can be blocked with SLC6A8 peptide (ab216175). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionRequired for the uptake of creatine in muscles and brain.
Tissue specificityPredominantly expressed in skeletal muscle and kidney. Also found in brain, heart, colon, testis and prostate.
Involvement in diseaseCerebral creatine deficiency syndrome 1
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A8 subfamily.