Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC7A5
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 16 amino acid synthetic peptide from near the N terminal residues of Human SLC7A5 protein (Swiss-Prot Q01650).

  • Positive control
    • Human lung lysate; human lung tissue.

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with SLC7A5 peptide (ab192836).
IHC-P 1/50 - 1/250.

Target

  • Function
    Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.
  • Tissue specificity
    Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.
  • Sequence similarities
    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization
    Cytoplasm > cytosol. Apical cell membrane. Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4F2 LC antibody
    • 4F2 light chain antibody
    • 4F2LC antibody
    • CD98 antibody
    • CD98 light chain antibody
    • CD98LC antibody
    • D16S469E antibody
    • DC49 antibody
    • E16 antibody
    • hLAT1 antibody
    • Integral membrane protein E16 antibody
    • L type amino acid transporter antibody
    • L type amino acid transporter 1 antibody
    • L-type amino acid transporter 1 antibody
    • Large neutral amino acids transporter 1 antibody
    • Large neutral amino acids transporter antibody
    • Large neutral amino acids transporter small subunit 1 antibody
    • LAT1 antibody
    • LAT1_HUMAN antibody
    • Membrane protein E16 antibody
    • MPE16 antibody
    • Slc7a5 antibody
    • Sodium independent neutral amino acid transporter antibody
    • Sodium independent neutral amino acid transporter LAT1 antibody
    • Solute carrier family 7 (cationic amino acid transporter y+ system) member 5 antibody
    • Solute carrier family 7 member 5 antibody
    • TA1 antibody
    • Tumor associated protein 1 antibody
    • y+ system cationic amino acid transporter antibody
    see all

Images

  • Anti-SLC7A5 antibody (ab85226) + Human SW480 (colorectal adenocarcinoma) cell line at 1/500 dilution

    Predicted band size : 55 kDa
  • Anti-SLC7A5 antibody (ab85226) at 1/200 dilution + human lung lysate

    Predicted band size : 55 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab85226 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85226, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab85226, at 1/100 dilution, staining SLC7A5 in formalin-fixed, paraffin-embedded human lung by Immunohistochemistry.

References

This product has been referenced in:
  • Guastella AR  et al. Tryptophan PET Imaging of the Kynurenine Pathway in Patient-Derived Xenograft Models of Glioblastoma. Mol Imaging 15: (2016). IHC . Read more (PubMed: 27151136) »
  • Reidy PT  et al. Soy-dairy protein blend and whey protein ingestion after resistance exercise increases amino acid transport and transporter expression in human skeletal muscle. J Appl Physiol (1985) 116:1353-64 (2014). WB ; Human . Read more (PubMed: 24699854) »

See all 6 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
80 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Miss. Dora Tarlungeanu

Verified customer

Submitted Dec 17 2015

Thank you for contacting us.

Yes, both ab85226 and ab134121 detect the same SLC7A5 (LAT1) protein. http://www.uniprot.org/uniprot/Q01650

We would guarantee ab134121 to work in WB, IP, and IHC-P with human samples. We know that it d...

Read More

Thanks for your inquiry. From all the information available, there is no preferred antigen retrieval step for ab85226. I have attached the standard antigen retrieval step for IHC as part of the IHC-P protocol. Please refer to the antigen retriev...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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