Overview

  • Product name
  • Description
    Goat polyclonal to SLC7A5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    C-RHRKPELERPIKVN

    , corresponding to internal sequence amino acids 419-432 of Human SLC7A5 (NP_003477.4).

  • Positive control
    • Human Spleen lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99419 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • Function
    Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.
  • Tissue specificity
    Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.
  • Sequence similarities
    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • Cellular localization
    Cytoplasm > cytosol. Apical cell membrane. Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiophoblastic cells. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4F2 LC antibody
    • 4F2 light chain antibody
    • 4F2LC antibody
    • CD98 antibody
    • CD98 light chain antibody
    • CD98LC antibody
    • D16S469E antibody
    • DC49 antibody
    • E16 antibody
    • hLAT1 antibody
    • Integral membrane protein E16 antibody
    • L type amino acid transporter antibody
    • L type amino acid transporter 1 antibody
    • L-type amino acid transporter 1 antibody
    • Large neutral amino acids transporter 1 antibody
    • Large neutral amino acids transporter antibody
    • Large neutral amino acids transporter small subunit 1 antibody
    • LAT1 antibody
    • LAT1_HUMAN antibody
    • Membrane protein E16 antibody
    • MPE16 antibody
    • Slc7a5 antibody
    • Sodium independent neutral amino acid transporter antibody
    • Sodium independent neutral amino acid transporter LAT1 antibody
    • Solute carrier family 7 (cationic amino acid transporter y+ system) member 5 antibody
    • Solute carrier family 7 member 5 antibody
    • TA1 antibody
    • Tumor associated protein 1 antibody
    • y+ system cationic amino acid transporter antibody
    see all

Images

  • All lanes : Anti-SLC7A5 antibody (ab99419) at 0.3 µg

    Lane 1 : Human Spleen lysate
    Lane 2 : Human Spleen lysate with immunizing peptide

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 55 kDa

References

ab99419 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

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Verified customer

Submitted Jan 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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