Our Abpromise guarantee covers the use of ab122727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labelling SLC7A6OS with ab122727 shows positivity in the nucleus but is excluded from the nucleoli.
ab122727 has not yet been referenced specifically in any publications.