Overview

  • Product nameAnti-SLC9A8 antibody
    See all SLC9A8 primary antibodies
  • Description
    Rabbit polyclonal to SLC9A8
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide from within internal sequence amino acids 531-580: AKYLNPFFTR RLTQEDLHHG RIQMKTLTNK WYEEVRQGPS GSEDDEQELL, of Human SLC9A8 (NP_056081)

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionInvolved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • Tissue specificityUbiquitous. Strongly expressed in skeletal muscle and kidney.
  • Sequence similaritiesBelongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Na(+)/H(+) exchanger 8 antibody
    • NHE 8 antibody
    • NHE-8 antibody
    • NHE8 antibody
    • SL9A8_HUMAN antibody
    • Slc9a8 antibody
    • Sodium/ hydrogen exchanger 8 antibody
    • Sodium/hydrogen exchanger 8 antibody
    • solute carrier family 9 (sodium/hydrogen exchanger), member 8 antibody
    • Solute carrier family 9 member 8 antibody
    see all

Anti-SLC9A8 antibody images

  • Anti-SLC9A8 antibody (ab83937) at 1 µg/ml (in 5% skim milk/ PBS buffer) + HepG2 cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SLC9A8 antibody (ab83937)

ab83937 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83937.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"