Overview

  • Product nameAnti-SLCO2B1 antibody
  • Description
    Rabbit polyclonal to SLCO2B1
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (DPQDVRPSVF HNIKLFVLCH SLLQLAQLMI SGYLKSSIST VEKRFGLSSQ) of Human SLCO2B1 (NP_009187)

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceSLCO2B1 (solute carrier organic anion transporter family member 2B1), or Organic anion transporter B (OATP-B) mediates sodium-independent transport of organic anions. Unlike other related proteins, SLCO2B1 is expressed in many tissues, including liver, placenta, brain, heart and intestine. SLCO2B1 plays a role in the uptake of steroids and a number of drugs such as statins.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Moat 1 antibody
    • Moat1 antibody
    • Multispecific organic anion transporter 1 antibody
    • Multispecific organic anion transporter1 antibody
    • OATP 2B1 antibody
    • OATP B antibody
    • OATP RP2 antibody
    • OATP2 B1 antibody
    • OATP2B1 antibody
    • OATPB antibody
    • OATPRP 2 antibody
    • OATPRP2 antibody
    • Organic anion transporter B antibody
    • Organic anion transporter polypeptide related protein 2 antibody
    • Organic anion transporter polypeptide related protein2 antibody
    • SLC 21A9 antibody
    • SLC2 1A9 antibody
    • SLC21 A9 antibody
    • SLC21A9 antibody
    • SLCO 2B1 antibody
    • SLCO2 B1 antibody
    • SO 2B1 antibody
    • SO2 B1 antibody
    • SO2B1 antibody
    • Solute carrier family 21 (organic anion transporter), member 9 antibody
    • Solute carrier family 21 member 9 antibody
    • Solute carrier organic anion transporter family member 2B1 antibody
    see all

Anti-SLCO2B1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling SLCO2B1 with ab83532 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SLCO2B1. Right - Merge.
  • Anti-SLCO2B1 antibody (ab83532) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Gel concentration: 12%

References for Anti-SLCO2B1 antibody (ab83532)

ab83532 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (overexpressed HEK293 cells)
Loading amount 60 µg
Specification overexpressed HEK293 cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"