Anti-SLD5 antibody (ab101346)
Key features and details
- Rabbit polyclonal to SLD5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SLD5 antibody
See all SLD5 primary antibodies -
Description
Rabbit polyclonal to SLD5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to amino acids 1-198 of Human SLD5
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Positive control
- 293T, A431, Jurkat cell lines
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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ICC/IF | (1) |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
Target
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Function
The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. -
Sequence similarities
Belongs to the GINS4/SLD5 family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84296 Human
- Entrez Gene: 109145 Mouse
- Entrez Gene: 290842 Rat
- Omim: 610611 Human
- SwissProt: A2VE40 Cow
- SwissProt: Q9BRT9 Human
- SwissProt: Q99LZ3 Mouse
- SwissProt: Q499W2 Rat
see all -
Alternative names
- DNA replication complex GINS protein SLD5 antibody
- GINS complex subunit 4 (Sld5 homolog) antibody
- GINS complex subunit 4 antibody
see all
Images
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Anti-SLD5 antibody (ab101346) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-SLD5 antibody (ab101346) at 1/10000 dilution + Mouse heart at 50 µg
Predicted band size: 26 kDa -
ab101346 at 1/500 staining SLD5 in paraffin-embedded human gastric cancer.
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ab101346 at 1/200 staining paraformaldehyde-fixed A431 in IF. Bottom image costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab101346 has been referenced in 3 publications.
- Zhang Z et al. Overexpression of GINS4 Is Associated With Tumor Progression and Poor Survival in Hepatocellular Carcinoma. Front Oncol 11:654185 (2021). PubMed: 33842367
- Kaur M et al. Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites. Mol Cell Biol 38:N/A (2018). PubMed: 29061732
- Kimura T et al. Induced expression of GINS complex is an essential step for reactivation of quiescent stem-like tumor cells within the peri-necrotic niche in human glioblastoma. J Cancer Res Clin Oncol N/A:N/A (2018). PubMed: 30465075