Overview

  • Product nameAnti-SLFN11 antibody
  • Description
    Rabbit polyclonal to SLFN11
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: REVLGCAKEN VDPDSLRRKI EQAIYKLPCV HFCQPQRPIT FTLKIVDVLK RGELYGYACM IRVNPFCCAV FSEAPNSWI, corresponding to amino acids 255-333 of Human SLFN11.

  • Positive control
    • Human pancreas tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-SLFN11 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121731 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Anti-SLFN11 antibody (ab121731) + MOLT-4

    Secondary
    SLFN11

    Predicted band size : 103 kDa
    Observed band size : 103 kDa

    Western blot analysis in human cell line MOLT-4 staining SLFN11 with ab121731

  • ab121731 at 1/15 dilution staining SLFN11 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in islet cells.

References for Anti-SLFN11 antibody (ab121731)

ab121731 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"