Anti-SLFN5 antibody (ab121537)
Key features and details
- Rabbit polyclonal to SLFN5
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLFN5 antibody -
Description
Rabbit polyclonal to SLFN5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to internal sequence amino acids 70-210 of Human SLFN5.
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Positive control
- ICC/IF: A-431 human cell line IHC: Human lymph node, Fallopian tube and endometrium tissue Wb: SK-MEL-30 and Caco-2 cell lines
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
May have a role in hematopoeitic cell differentiation. -
Sequence similarities
Belongs to the Schlafen family. - Information by UniProt
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Database links
- Entrez Gene: 162394 Human
- SwissProt: Q08AF3 Human
- Unigene: 709347 Human
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Alternative names
- AI956840 antibody
- RP23-392I13.5 antibody
- Schlafen 5 antibody
see all
Images
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ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human fallopian tube by Immunohistochemistry.
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ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human endometrium by Immunohistochemistry.
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ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human lymph node by Immunohistochemistry.
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Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-SLFN5 antibody (ab121537) at 0.4 µg/ml
Lane 1 : SK-MEL-30 cell lysate
Lane 2 : Caco-2 cell lysateLoading control: Anti-HDAC1
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab121537 has been referenced in 3 publications.
- Martinez RS et al. SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer. Cancer Res 81:3664-3678 (2021). PubMed: 33985973
- Companioni Nápoles O et al. SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer. J Gastroenterol 52:39-49 (2017). PubMed: 27032393
- Ding L et al. Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia. J Clin Invest 126:2867-80 (2016). PubMed: 27427984