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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Anti-SLFN5 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-SLFN5 antibody (ab121537)
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)
ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human nasopharynx by Immunohistochemistry.
Western blot - Anti-SLFN5 antibody (ab121537)
All lanes : Anti-SLFN5 antibody (ab121537) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"