Overview

  • Product nameAnti-SLIRP antibody [1E10BC8]
    See all SLIRP primary antibodies
  • Description
    Mouse monoclonal [1E10BC8] to SLIRP
  • Tested applicationsSuitable for: WB, In-Cell ELISA, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Partial recombinant human SLIRP protein

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab119687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceSLIRP is a RNA binding protein that acts as a nuclear receptor corepressor. It may act by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. SLIRP binds the STR7 loop of SRA RNA. It is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It contains 1 RRM (RNA recognition motif) domain. SLIRP is predominantly founs in mitochondrial with a limited role in the nucleus where it is recruited to nuclear receptor target promoters.
  • Cellular localizationMitochondrial and Nuclear; Predominantly mitochondrial.
  • Database links
  • Alternative names
    • C14orf156 antibody
    • Chromosome 14 open reading frame 156 antibody
    • DC50 antibody
    • PD04872 antibody
    • SLIRP antibody
    • SRA stem loop interacting RNA binding protein, mitochondrial antibody
    see all

Anti-SLIRP antibody [1E10BC8] images

  • All lanes : Anti-SLIRP antibody [1E10BC8] (ab119687) at 1 µg/ml

    Lane 1 : HepG2 lysate at 20 µg
    Lane 2 : fibroblast lysate at 20 µg/ml
    Lane 3 : SLIRP recombinant protein at 0.01 µg

    Secondary
    Infra red fluorescence - Goat anti-mouse 800 at 1/5000 dilution

    Predicted band size : 12 kDa
  • Immunocytochemistry of ab119687 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab119687, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
  • Fibroblast (HDFn) cells were stained with 1µg/mL SLIRP antibody (ab119687) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • IHC image of SLIRP staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119687, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SLIRP antibody [1E10BC8] (ab119687)

ab119687 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"