Anti-SLIRP antibody (ab51523)
Key features and details
- Rabbit polyclonal to SLIRP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLIRP antibody
See all SLIRP primary antibodies -
Description
Rabbit polyclonal to SLIRP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Synthetic peptide made to a C terminal region of human SLIRP.
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Positive control
- ICC/IF: HeLa, U2OS cells. IHC-P: Mouse intestine tissue. WB: Human liver lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, Tris glycine -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/10 - 1/500.
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IHC-P |
1/100.
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WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
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Notes |
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ICC/IF
1/10 - 1/500. |
IHC-P
1/100. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
Target
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Relevance
SLIRP is a RNA binding protein that acts as a nuclear receptor corepressor. It may act by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. SLIRP binds the STR7 loop of SRA RNA. It is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It contains 1 RRM (RNA recognition motif) domain. SLIRP is predominantly founs in mitochondrial with a limited role in the nucleus where it is recruited to nuclear receptor target promoters. -
Cellular localization
Mitochondrial and Nuclear; Predominantly mitochondrial. -
Database links
- Entrez Gene: 81892 Human
- Omim: 610211 Human
- SwissProt: Q9GZT3 Human
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Alternative names
- C14orf156 antibody
- Chromosome 14 open reading frame 156 antibody
- DC50 antibody
see all
Images
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U2OS cells stained for SLIRP (green) using ab51523 at 1/500 dilution in immunocytochemistry. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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HeLa cells stained for SLIRP using ab51523 at 1/200 dilution overnight at 4°C and detected with an anti-rabbit Dylight 488 (green) at 1/500 dilution in immunocytochemistry. Alpha tubulin was used as a co-stain at a 1/1000 dilution and detected with and anti-mouse Dylight 550 (red) at 1/500 dilution. Nuclei were counterstained with DAPI (blue).
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Anti-SLIRP antibody (ab51523) at 0.6 µg/ml + human liver lysate
Predicted band size: 12 kDa
Observed band size: 12 kDa
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab51523 has been referenced in 8 publications.
- De Silva D et al. Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen. Sci Rep 9:18637 (2019). PubMed: 31819114
- Reyes A et al. RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. Front Genet 10:1393 (2019). PubMed: 32082360
- Carpp LN et al. Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection. Mol Cell Proteomics 13:2836-54 (2014). PubMed: 24855065
- Clemente P et al. hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria. J Biol Chem 288:8321-31 (2013). PubMed: 23362268
- Bayat V et al. Mutations in the Mitochondrial Methionyl-tRNA Synthetase Cause a Neurodegenerative Phenotype in Flies and a Recessive Ataxia (ARSAL) in Humans. PLoS Biol 10:e1001288 (2012). WB ; Human . PubMed: 22448145
- Ebmeier CC & Taatjes DJ Activator-Mediator binding regulates Mediator-cofactor interactions. Proc Natl Acad Sci U S A 107:11283-8 (2010). WB ; Human . PubMed: 20534441
- Sasarman F et al. LRPPRC and SLIRP interact in a ribonucleoprotein complex that regulates posttranscriptional gene expression in mitochondria. Mol Biol Cell 21:1315-23 (2010). WB ; Human . PubMed: 20200222
- Abedin SA et al. Elevated NCOR1 disrupts a network of dietary-sensing nuclear receptors in bladder cancer cells. Carcinogenesis 30:449-56 (2009). WB ; Human . PubMed: 19126649