Overview

  • Product nameAnti-Slit1 antibody
    See all Slit1 primary antibodies
  • Description
    Mouse polyclonal to Slit1
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Mouse Slit1 aa 497-504 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    CSAKEQYFIPGTEDYHLN

  • Positive control
    • IHC-P: Human Kidney tissue; Human Pancreas tissue
  • General notesAvoid repeated freezing and thawing.

Properties

Applications

Our Abpromise guarantee covers the use of ab115892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 167 kDa.
ELISA 1/10000 - 1/50000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionThought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
  • Tissue specificityPredominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 9 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0813 antibody
    • MEGF 4 antibody
    • MEGF4 antibody
    • MGC164811 antibody
    • Multiple EGF-like domains protein 4 antibody
    • Multiple epidermal growth factor like domains 4 antibody
    • Multiple epidermal growth factor-like domains protein 4 antibody
    • OTTHUMP00000059392 antibody
    • SLIL 1 antibody
    • SLIL1 antibody
    • Slit 1 antibody
    • SLIT 3 antibody
    • Slit homolog 1 antibody
    • Slit homolog 1 protein antibody
    • Slit-1 antibody
    • Slit1 antibody
    • SLIT1_HUMAN antibody
    • SLIT3 antibody
    see all

Anti-Slit1 antibody images

  • ab115892, at 5 ug/ml, staining Slit1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. Developed using Goat anti rabbit biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab115892, at 5 ug/ml, staining Slit1 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry. Developed using Goat anti rabbit biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-Slit1 antibody (ab115892)

ab115892 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris (MOPS buffer))
Sample Human Cell lysate - whole cell (HEK293T with construct expressing human Slit1-myc)
Specification HEK293T with construct expressing human Slit1-myc
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 51% · Temperature: RT°C
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Submitted Dec 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"