- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Slit1 antibody [EP5796(N)]
See all Slit1 primary antibodies -
DescriptionRabbit monoclonal [EP5796(N)] to Slit1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Slit1 aa 50-150. The exact sequence is proprietary.
Database link: O75093 -
Positive control
- Human fetal brain and forebrain lysates; SH-SY5Y cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP5796(N)
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab183704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 200 kDa (predicted molecular weight: 168 kDa). |
Target
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FunctionThought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
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Tissue specificityPredominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.
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Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 6585 Human
- Omim: 603742 Human
- SwissProt: O75093 Human
- Unigene: 632082 Human
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Alternative names
- KIAA0813 antibody
- MEGF 4 antibody
- MEGF4 antibody
see all
Images
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![Western blot - Anti-Slit1 [EP5796(N)] antibody (ab183704)](http://a.abcam.com/ps/products/183/ab183704/Images/ab183704-215817-wb.jpg)
Western blot - Anti-Slit1 [EP5796(N)] antibody (ab183704)Anti-Slit1 antibody [EP5796(N)] (ab183704) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 168 kDa -
![Western blot - Anti-Slit1 [EP5796(N)] antibody (ab183704)](http://a.abcam.com/ps/products/183/ab183704/Images/ab183704-215816-wb.jpg)
Western blot - Anti-Slit1 [EP5796(N)] antibody (ab183704)All lanes : Anti-Slit1 antibody [EP5796(N)] (ab183704) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal forebrain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 168 kDa
Protocols
References
ab183704 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"