Overview

  • Product nameAnti-Slit2 antibody
    See all Slit2 primary antibodies
  • Description
    Rabbit polyclonal to Slit2
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Slit2 (C terminal).
    Database link: 9353

  • Positive control
    • Human prostate adenocarcinoma tissue and Human fetal kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab111128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. For antigen retrieval, boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

Target

  • FunctionThought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
  • Tissue specificityFetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 7 EGF-like domains.
    Contains 1 laminin G-like domain.
    Contains 20 LRR (leucine-rich) repeats.
    Contains 4 LRRCT domains.
    Contains 4 LRRNT domains.
  • DomainThe leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
  • Cellular localizationSecreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Drad 1 antibody
    • E030015M03Rik antibody
    • E130320P19Rik antibody
    • FLJ14420 antibody
    • OTTHUMP00000158695 antibody
    • OTTHUMP00000217852 antibody
    • OTTHUMP00000217853 antibody
    • OTTHUMP00000217854 antibody
    • Slil 3 antibody
    • Slil3 antibody
    • Slit 2 antibody
    • Slit homolog 2 (Drosophila) antibody
    • Slit homolog 2 antibody
    • Slit homolog 2 protein antibody
    • Slit homolog 2 protein C-product antibody
    • Slit-2 antibody
    • Slit2 antibody
    • SLIT2_HUMAN antibody
    see all

Anti-Slit2 antibody images

  • ab111128 at 1/100 dilution staining Slit2 in Formalin-fixed, paraffin-embedded Human prostate adenocarcinoma tissue.

References for Anti-Slit2 antibody (ab111128)

ab111128 has not yet been referenced specifically in any publications.

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