Synthetic peptide:
YFIPGTEDYRSKLSGDC
conjugated to KLH, corresponding to amino acids 484-500 of Human SLIT2.Our Abpromise guarantee covers the use of ab7665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | Use at an assay dependent concentration. | |
WB | 1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal. | |
IHC-Fr | Use at an assay dependent concentration. PubMed: 20498081 |
Molecular weight estimation was made by comparison to prestained molecular weight markers
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"