- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Slit2 antibody
See all Slit2 primary antibodies -
DescriptionRabbit polyclonal to Slit2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Xenopus laevis
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Immunogen
Synthetic peptide:
YFIPGTEDYRSKLSGDC
conjugated to KLH, corresponding to amino acids 484-500 of Human SLIT2. -
Positive control
- Chicken spinal cord whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab7665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20498081 |
Target
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FunctionThought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
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Tissue specificityFetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.
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Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 7 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains. -
DomainThe leucine-rich repeat domain is sufficient for guiding both axon projection and neuronal migration, in vitro.
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Cellular localizationSecreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.
- Information by UniProt
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Database links
- Entrez Gene: 373967 Chicken
- Entrez Gene: 9353 Human
- Entrez Gene: 20563 Mouse
- Entrez Gene: 360272 Rat
- Entrez Gene: 394404 Xenopus laevis
- Omim: 603746 Human
- SwissProt: O94813 Human
- SwissProt: Q9R1B9 Mouse
see all -
Alternative names
- Drad 1 antibody
- E030015M03Rik antibody
- E130320P19Rik antibody
see all
Images
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Western blot - Anti-Slit2 antibody (ab7665)Anti-Slit2 antibody (ab7665) at 1/1350 dilution + Chicken spinal cord whole cell lysate at 30 µg
Secondary
IRDye™800
conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 170 kDa
Observed band size: 165 kDa (why is the actual band size different from the predicted?)Molecular weight estimation was made by comparison to prestained molecular weight markers
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Immunocytochemistry/ Immunofluorescence - Anti-Slit2 antibody (ab7665)Image courtesy of an anonymous Abreview.ab7665 staining Slit2 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab7665 at a 1/80 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI. -
Immunocytochemistry/ Immunofluorescence - Anti-Slit2 antibody (ab7665)Image courtesy of an anonymous Abreview.ab7665 staining Slit2 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab7665 at a 1/80 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI. -
Immunocytochemistry/ Immunofluorescence - Anti-Slit2 antibody (ab7665)Image courtesy of an anonymous Abreview.ab7665 staining Slit2 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab7665 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.
Protocols
References
This product has been referenced in:
- Wu H et al. Slit2 as a ß-catenin/Ctnnb1-dependent retrograde signal for presynaptic differentiation. Elife 4:N/A (2015). WB . Read more (PubMed: 26159615) »
- Cai H et al. Roundabout 4 regulates blood-tumor barrier permeability through the modulation of ZO-1, Occludin, and Claudin-5 expression. J Neuropathol Exp Neurol 74:25-37 (2015). Read more (PubMed: 25470344) »
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