Overview

  • Product nameAnti-SLK antibody
    See all SLK primary antibodies
  • Description
    Rabbit polyclonal to SLK
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminus of human SLK.

  • Positive control
    • Extracts from COLO205 cells, human skeletal muscle tissue, HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
IHC-P 1/50 - 1/100.

Target

  • RelevanceSLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CTCL tumor antigen se20 9 antibody
    • LOSK antibody
    • se20 9 antibody
    • Serine/threonine protein kinase 2 antibody
    • SLK antibody
    • SNF1 sucrose nonfermenting like kinase antibody
    • STE20 like serine/threonine-protein kinase antibody
    • STE20 related kinase antibody
    • STK2 antibody
    see all

Anti-SLK antibody images

  • This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.
  • All lanes : Anti-SLK antibody (ab65113) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells
    Lane 2 : extracts from COLO205 cells with immunising peptide


    Predicted band size : 143 kDa
    Observed band size : 143 kDa
  • Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution

References for Anti-SLK antibody (ab65113)

This product has been referenced in:
  • Müller T  et al. ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients. Oncogene : (2010). WB ; Human . Read more (PubMed: 20154719) »

See 1 Publication for this product

Product Wall

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Comme convenu j'ai mis en place l'envoi d'une unité de ab65113 en remplaceme...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela Cells)
Loading amount 20 µg
Specification Hela Cells
Gel Running Conditions Non-reduced Denaturing (7.5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"