Overview

  • Product name
  • Description
    Rabbit polyclonal to SLK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human SLK (NP_055535.2).

  • Positive control
    • MCF7, HeLa,and 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 143 kDa).
IP Use at 2 µg/mg of lysate.

Target

  • Relevance
    SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • CTCL tumor antigen se20 9 antibody
    • LOSK antibody
    • se20 9 antibody
    • Serine/threonine protein kinase 2 antibody
    • SLK antibody
    • SNF1 sucrose nonfermenting like kinase antibody
    • STE20 like serine/threonine-protein kinase antibody
    • STE20 related kinase antibody
    • STK2 antibody
    see all

Images

  • All lanes : Anti-SLK antibody (ab70230) at 1 µg/ml

    Lane 1 : MCF7 whole cell lysate at 50 µg
    Lane 2 : MCF7 whole cell lysate at 15 µg
    Lane 3 : MCF7 whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 15 µg
    Lane 5 : 293T whole cell lysate at 15 µg


    Predicted band size : 143 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
  • 250µg MCF7 whole cell lysate immunoprecipitated with ab70230 at 3 µg/mg lysate and immunoblotted at 1µg/ml. The band at 50-60 kDa is due to the heavy chain of the antibody.

References

ab70230 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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