Anti-Slow Skeletal Myosin Heavy chain antibody [M14] (ab97715)

Overview

  • Product name
    Anti-Slow Skeletal Myosin Heavy chain antibody [M14]
    See all Slow Skeletal Myosin Heavy chain primary antibodies
  • Description
    Mouse monoclonal [M14] to Slow Skeletal Myosin Heavy chain
  • Specificity
    This antibody does not react with SDS denatured myosin but reacts positively with non-denatured slow myosin.
  • Tested applications
    Suitable for: IHC-Fr, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Full length native protein (purified) (Human)

  • Positive control
    • Slow Skeletal Muscle.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    M14
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Function
    Muscle contraction.
  • Tissue specificity
    Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).
  • Involvement in disease
    Defects in MYH7 are the cause of cardiomyopathy familial hypertrophic type 1 (CMH1) [MIM:192600]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYH7 are the cause of myopathy myosin storage (MYOMS) [MIM:608358]. In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers.
    Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM) [MIM:181430]; also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm.
    Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S) [MIM:613426]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in MYH7 are the cause of myopathy distal type 1 (MPD1) [MIM:160500]. MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta myosin heavy chain antibody
    • cardiac muscle beta isoform antibody
    • CMD1S antibody
    • CMH1 antibody
    • MPD1 antibody
    • MYH7 antibody
    • MYH7_HUMAN antibody
    • Myhc slow antibody
    • MyHC-beta antibody
    • MyHC-slow antibody
    • MYHCB antibody
    • Myopathy, distal 1 antibody
    • Myosin heavy chain (AA 1-96) antibody
    • Myosin heavy chain 7 antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain slow isoform antibody
    • Myosin heavy chain, cardiac muscle beta isoform antibody
    • Myosin, heavy chain 7, cardiac muscle, beta antibody
    • Myosin, heavy polypeptide 7, cardiac muscle, beta antibody
    • Myosin-7 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.7A antibody
    • SPMD antibody
    • SPMM antibody
    see all

References

This product has been referenced in:
  • Dhoot GK Mammalian myoblasts become fast or slow myocytes within the somitic myotome. J Muscle Res Cell Motil 15:617-22 (1994). Read more (PubMed: 7706418) »

See 1 Publication for this product

Customer reviews and Q&As

DISCOUNT CODE: *********- For ab105439
DISCOUNT CODE: *******- For ab97715
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab105439 & ab97715in Flow Cytometry. This code will...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up