Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

Overview

  • Product nameAnti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D]
    See all Slow Skeletal Myosin Heavy chain primary antibodies
  • Description
    Mouse monoclonal [NOQ7.5.4D] to Slow Skeletal Myosin Heavy chain
  • Tested applicationsSuitable for: ELISA, RIA, IHC-P, Electron Microscopy, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Human Slow Skeletal Myosin Heavy chain. Human skeletal muscle myosin purified from myofibrils.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR201056, GR231200, GR285981. Lot numbers other than GR201056, GR231200, GR285981will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Use at an assay dependent concentration.

RIA Use at an assay dependent concentration.

Use at an assay dependent concentration.

IHC-P 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Electron Microscopy Use at an assay dependent concentration.

Use at an assay dependent concentration.

WB 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).

Use at an assay dependent concentration.

ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMuscle contraction.
  • Tissue specificityBoth wild type and variant Gln-403 are detected in skeletal muscle (at protein level).
  • Involvement in diseaseDefects in MYH7 are the cause of cardiomyopathy familial hypertrophic type 1 (CMH1) [MIM:192600]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in MYH7 are the cause of myopathy myosin storage (MYOMS) [MIM:608358]. In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers.
    Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM) [MIM:181430]; also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm.
    Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S) [MIM:613426]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in MYH7 are the cause of myopathy distal type 1 (MPD1) [MIM:160500]. MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta myosin heavy chain antibody
    • cardiac muscle beta isoform antibody
    • CMD1S antibody
    • CMH1 antibody
    • MPD1 antibody
    • MYH7 antibody
    • MYH7_HUMAN antibody
    • Myhc slow antibody
    • MyHC-beta antibody
    • MyHC-slow antibody
    • MYHCB antibody
    • Myopathy, distal 1 antibody
    • Myosin heavy chain (AA 1-96) antibody
    • Myosin heavy chain 7 antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain slow isoform antibody
    • Myosin heavy chain, cardiac muscle beta isoform antibody
    • Myosin, heavy chain 7, cardiac muscle, beta antibody
    • Myosin, heavy polypeptide 7, cardiac muscle, beta antibody
    • Myosin-7 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.7A antibody
    • SPMD antibody
    • SPMM antibody
    see all

Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] images

  • ab11083 at 1/500 dilution, staining Slow Skeletal Myosin Heavy chain in mouse tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

    See Abreview

  • ab11083 at 1/4000 dilution, staining Slow Skeletal Myosin Heavy chain in rabbit tongue tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

  • Lane 1 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 1/5000 dilution
    Lane 2 : Negative Control

    Lane 1 : Rat skeletal muscle whole cell lysate
    Lane 2 : Rat skeletal muscle whole cell lysate

    Secondary
    Goat Anti-Mouse IgG-Peroxidase

    Predicted band size : 200 kDa
  • Lane 1 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 1/5000 dilution
    Lane 2 : Negative Control (Secondary only)

    Lane 1 : Whole cell extract from rabbit tongue
    Lane 2 : Whole cell extract from rabbit tongue


    Predicted band size : 200 kDa

References for Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)

This product has been referenced in:
  • Larsen S  et al. Increased intrinsic mitochondrial respiratory capacity in skeletal muscle from rats with streptozotocin-induced hyperglycemia. Physiol Rep 3:N/A (2015). WB ; Rat . Read more (PubMed: 26197936) »
  • Burrows AM  et al. Human faces are slower than chimpanzee faces. PLoS One 9:e110523 (2014). IHC-P ; Human . Read more (PubMed: 25338058) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Sand Lizard (Lacetra agilis) Tissue sections (muscle)
Permeabilization Yes - 0,1% Tween-20/PBS
Specification muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Aug 24 2016

Application Immunohistochemistry (Frozen sections)
Sample Grass snake (Natrix natrix) Tissue sections (skeletal muscle)
Permeabilization Yes - 0,1% Tween-20/PBS
Specification skeletal muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Aug 24 2016

Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (muscle)
Permeabilization No
Specification muscle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Methanol
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Submitted Oct 13 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (muscle)
Specification muscle
Permeabilization No
Fixative Formaldehyde
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Submitted Oct 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Other
Sample Mouse Tissue sections (soleus muscle)
Specification soleus muscle
Permeabilization No
Fixative Bouin fixation
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Submitted Oct 23 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"