Overview

  • Product name
  • Description
    Rabbit polyclonal to SLP76
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein:

    HEEPS RSRNHKTAKL PAPSIDRSTK PPLDRSLAPF DREPFTLGKK PPFSDKPSIP AGRSLGEHLP KIQKPPLPPT TERHERSSPL PGKKPPVPKH GWGPDRREND EDDVHQRPLP QPALLPMSSN TFPSRSTKPS PMNPLPSSHM PGAFSESNSS FPQSASLPPY FSQGPSNRPP IRAEGRNFPL PLPNKPRPPS PAEEENSLNE EWYVSYITRP EAEA

    , corresponding to Internal sequence amino acids 216-434 of Human SLP76.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from rabbit antisera by precipitation methods.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 64 kDa.

Target

  • Function
    Involved in T-cell antigen receptor mediated signaling.
  • Tissue specificity
    Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
  • Sequence similarities
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH2 domain.
  • Domain
    The SH2 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated after T-cell receptor activation by ZAP-70.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 76 kDa tyrosine phosphoprotein antibody
    • CG8697 antibody
    • LCP 2 antibody
    • LCP2 antibody
    • LCP2_HUMAN antibody
    • Lymphocyte cytosolic protein 2 antibody
    • SH2 domain containing leukocyte protein 76 KD antibody
    • SH2 domain containing leukocyte protein of 76kD antibody
    • SH2 domain containing leukocyte protein of 76kDa antibody
    • SH2 domain-containing leukocyte protein of 76 kDa antibody
    • SLP 76 antibody
    • SLP 76 tyrosine phosphoprotein antibody
    • SLP-76 antibody
    • SLP-76 tyrosine phosphoprotein antibody
    • SLP76 antibody
    • SLP76 tyrosine phosphoprotein antibody
    see all

References

This product has been referenced in:
  • Gill T & Levine AD Mitochondria-derived hydrogen peroxide selectively enhances T cell receptor-initiated signal transduction. J Biol Chem 288:26246-55 (2013). WB ; Human . Read more (PubMed: 23880762) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (jurkats)
Specification
jurkats
Treatment
TCR activation with OKT3 and anti CD28
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Tejpal Gill

Verified customer

Submitted Jan 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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