Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab17029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).

Target

  • FunctionInvolved in T-cell antigen receptor mediated signaling.
  • Tissue specificityHighly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
  • Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH2 domain.
  • DomainThe SH2 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated after T-cell receptor activation by ZAP-70.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 76 kDa tyrosine phosphoprotein antibody
    • CG8697 antibody
    • LCP 2 antibody
    • LCP2 antibody
    • LCP2_HUMAN antibody
    • Lymphocyte cytosolic protein 2 antibody
    • SH2 domain containing leukocyte protein 76 KD antibody
    • SH2 domain containing leukocyte protein of 76kD antibody
    • SH2 domain containing leukocyte protein of 76kDa antibody
    • SH2 domain-containing leukocyte protein of 76 kDa antibody
    • SLP 76 antibody
    • SLP 76 tyrosine phosphoprotein antibody
    • SLP-76 antibody
    • SLP-76 tyrosine phosphoprotein antibody
    • SLP76 antibody
    • SLP76 tyrosine phosphoprotein antibody
    see all

Anti-SLP76 antibody images

  • Anti-SLP76 antibody (ab17029) at 0.3 µg/ml + Jurkat lysate (protein in RIPA buffer) at 35 µg

    Predicted band size : 61 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-SLP76 antibody (ab17029)

ab17029 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Goat Cell lysate - whole cell (Jurkat)
Loading amount 1e+006 cells
Specification Jurkat
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Dec 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"