Anti-SLP76 antibody [EPR2549(2)] (ab109254)

Overview

  • Product nameAnti-SLP76 antibody [EPR2549(2)]
    See all SLP76 primary antibodies
  • Description
    Rabbit monoclonal [EPR2549(2)] to SLP76
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of Human SLP76.

  • Positive control
    • MOLT4 or HuT78 cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR2549(2)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionInvolved in T-cell antigen receptor mediated signaling.
    • Tissue specificityHighly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
    • Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH2 domain.
    • DomainThe SH2 domain mediates interaction with SHB.
    • Post-translational
      modifications
      Phosphorylated after T-cell receptor activation by ZAP-70.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 76 kDa tyrosine phosphoprotein antibody
      • CG8697 antibody
      • LCP 2 antibody
      • LCP2 antibody
      • LCP2_HUMAN antibody
      • Lymphocyte cytosolic protein 2 antibody
      • SH2 domain containing leukocyte protein 76 KD antibody
      • SH2 domain containing leukocyte protein of 76kD antibody
      • SH2 domain containing leukocyte protein of 76kDa antibody
      • SH2 domain-containing leukocyte protein of 76 kDa antibody
      • SLP 76 antibody
      • SLP 76 tyrosine phosphoprotein antibody
      • SLP-76 antibody
      • SLP-76 tyrosine phosphoprotein antibody
      • SLP76 antibody
      • SLP76 tyrosine phosphoprotein antibody
      see all

    Anti-SLP76 antibody [EPR2549(2)] images

    • All lanes : Anti-SLP76 antibody [EPR2549(2)] (ab109254) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : HuT78 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 60 kDa
    • ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109254 showing positive staining in Normal colon tissue.

    • ab109254 showing negative staining in Normal brain tissue.

    • ab109254 showing negative staining in Normal heart tissue.

    • ab109254 showing positive staining in Normal spleen tissue.

    References for Anti-SLP76 antibody [EPR2549(2)] (ab109254)

    ab109254 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab109254.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"