The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC,IHC-P or IP.
Involved in T-cell antigen receptor mediated signaling.
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Cimo AM et al. CD25 and CD69 induction by a4ß1 outside-in signalling requires TCR early signalling complex proteins. Biochem J454:109-21 (2013).
Read more (PubMed: 23758320) »
Köhler K et al. Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells. PLoS One5:e15374 (2010).
Read more (PubMed: 21179506) »