Overview

  • Product nameAnti-SLPI antibody [31]
    See all SLPI primary antibodies
  • Description
    Mouse monoclonal [31] to SLPI
  • SpecificityThe reactivity of the antibody with the isolated domains was evaluated using isolated domains of SLPI obtained by cleavage using partial acidic hydrolysis. Therefore, it is possible that the antibody will recognize other SLPI cleavage products generated by e.g. (microbial) proteases.
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human antileukoprotease (ALP).

  • Positive control
    • PMN lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab17157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
WB 1/50. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa). If bands larger than 12-14 kDa are seen then this can be explained by aggregation of SLPI. Due to cationic character of SLPI it is also possible that SLPI gives a band at 14 kDa instead of 12 kDa.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionAcid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.
  • Tissue specificityMucous fluids.
  • Sequence similaritiesContains 2 WAP domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALK 1 antibody
    • ALK1 antibody
    • ALP antibody
    • Antileukoprotease antibody
    • Antileukoproteinase 1 antibody
    • Antileukoproteinase antibody
    • Antileukoproteinase1 antibody
    • BLPI antibody
    • Human seminal protease inhibitor antibody
    • HUSI 1 antibody
    • HUSI antibody
    • HUSI I antibody
    • HUSI-1 antibody
    • MPI antibody
    • Mucus proteinase inhibitor antibody
    • Protease inhibitor WAP4 antibody
    • Secretory leukocyte peptidase inhibitor antibody
    • Secretory leukocyte protease inhibitor antibody
    • Seminal proteinase inhibitor antibody
    • SLPI antibody
    • SLPI_HUMAN antibody
    • WAP four disulfide core domain protein 4 antibody
    • WAP four-disulfide core domain protein 4 antibody
    • WAP4 antibody
    • WFDC4 antibody
    see all

Anti-SLPI antibody [31] images

  • ab17157 staining SLPI in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the HOPE technique and blocked for 15 minutes. Samples were incubated with primary antibody (1/4000) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-SLPI antibody [31] (ab17157)

ab17157 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Zytomed Systems Polymer Kit Blocking Solution as blocking agent for 15 minute(s) · Concentration: 100%
Antigen retrieval step None
Sample Human Tissue sections (Lung cancer)
Specification Lung cancer
Permeabilization No
Fixative Hope-Technique
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Submitted Jun 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"