A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.
Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.
Western blot - SLPI antibody - Mid molecule (ab46763)
All lanes : Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1 : Recombinant Human SLP I at 0.04 µg Lane 2 : Recombinant Human SLP I at 0.02 µg Lane 3 : Recombinant Human SLP I at 0.01 µg Lane 4 : Recombinant Human SLP I at 0.004 µg Lane 5 : Recombinant Human SLP I at 0.001 µg
Klimenkova O et al. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. Blood123:1239-49 (2014).
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Yang Y et al. Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker. PLoS One9:e95389 (2014).
Read more (PubMed: 24748380) »