Overview

  • Product name
  • Description
    Rabbit polyclonal to SLU7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 349-586 of Human SLU7 (Uniprot O95391).

  • Positive control
    • Lysate of 293T cells transfected with SLU7 recombinant protein; A431 cells, Human lung adenocarcinoma tissue.
  • General notes
    Store as concentrated solution

Properties

Applications

Our Abpromise guarantee covers the use of ab151462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
  • Sequence similarities
    Belongs to the SLU7 family.
    Contains 1 CCHC-type zinc finger.
  • Domain
    The CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9G8 antibody
    • hSlu7 antibody
    • MGC9280 antibody
    • Pre mRNA splicing factor SLU7 antibody
    • Pre-mRNA-splicing factor slu7 antibody
    • SLU 7 antibody
    • slu7 antibody
    • SLU7 splicing factor homolog (S. cerevisiae) antibody
    • SLU7 splicing factor homolog antibody
    • SLU7_HUMAN antibody
    • splicing factor antibody
    • Step II splicing factor SLU7 antibody
    • Zinc knuckle motif containing antibody
    see all

Images

  • All lanes : Anti-SLU7 antibody (ab151462) at 1/10000 dilution

    Lane 1 : Non-transfected 293T lysates
    Lane 2 : Human SLU7 transfected 293T lysates

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells, labelling SLU7 with ab151462 at 1/500 dilution. The lower image shows ab151462 staining (green) overlaid with Hoechst 33342 (blue).
  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue, labelling SLU7 with ab151462 at 1/250 dilution.

References

ab151462 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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