Anti-SLUG antibody (ab106077)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLUG antibody
See all SLUG primary antibodies -
Description
Rabbit polyclonal to SLUG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SLUG aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab133782) -
General notes
This antibody has only been tested on overexpressing cell lysates; we have no data on the detection of the endogenous protein.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Omim: 602150 Human
- SwissProt: O43623 Human
- SwissProt: P97469 Mouse
- Unigene: 360174 Human
- Unigene: 4272 Mouse
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Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Images
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All lanes : Anti-SLUG antibody (ab106077) at 1 µg/ml
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 cell lysate overexpressing SNAIL protein
Lane 3 : MCF7 cell lysate transfected with vector control plasmid
Lane 4 : Untransfected MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
All lanes : Anti-SLUG antibody (ab106077) at 1/2000 dilution
Lane 1 : MDA-MB-231 whole cell lysate
Lane 2 : MDA-MB-231 whole cell lysate - sh Slug 1
Lane 3 : MDA-MB-231 whole cell lysate - sh Slug 2
Lane 4 : MDA-MB-231 whole cell lysate - sh Snail 1
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 35,65 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 22°C.
Primary incubation at 4°C for 18 hours.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab106077 has been referenced in 23 publications.
- Liu W et al. miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition. Front Oncol 11:611544 (2021). PubMed: 33937023
- He DH et al. Phytochemical library screening reveals betulinic acid as a novel Skp2-SCF E3 ligase inhibitor in non-small cell lung cancer. Cancer Sci 112:3218-3232 (2021). PubMed: 34080260
- Tian C et al. Linc-ROR facilitates progression and angiogenesis of hepatocellular carcinoma by modulating DEPDC1 expression. Cell Death Dis 12:1047 (2021). PubMed: 34741030
- Yang Y et al. Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2. Onco Targets Ther 13:2247-2260 (2020). PubMed: 32214825
- Tian S et al. SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/ß-catenin signaling pathway. Aging (Albany NY) 12:3574-3593 (2020). PubMed: 32091407