Overview

  • Product name
  • Description
    Rabbit polyclonal to SLX4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human SLX4.

    (Peptide available as ab116498.)

  • Positive control
    • This antibody gave a positive signal in HEK293 and U2OS whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab100997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).

Target

  • Function
    Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks.
  • Sequence similarities
    Belongs to the SLX4 family.
    Contains 1 BTB (POZ) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Localizes to sites of DNA dammage.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTB (POZ) domain containing 12 antibody
    • BTB/POZ domain-containing protein 12 antibody
    • BTBD12 antibody
    • FANCP antibody
    • KIAA1784 antibody
    • KIAA1987 antibody
    • MUS312 antibody
    • SLX 4 antibody
    • slx4 antibody
    • SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae) antibody
    • SLX4_HUMAN antibody
    • Structure-specific endonuclease subunit slx4 antibody
    see all

Images

  • All lanes : Anti-SLX4 antibody (ab100997) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 200 kDa
    Observed band size: 200 kDa
    Additional bands at: 175 kDa (possible isoform), 42 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 2 of SLX4. The predicted molecular weights of isoforms 1 and 2 are 200 kDa and 166 kDa respectively.

References

ab100997 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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