Anti-SLX4 antibody (ab169114)
Key features and details
- Mouse polyclonal to SLX4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLX4 antibody -
Description
Mouse polyclonal to SLX4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SLX4. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- SLX4 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 127 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 127 kDa. |
Target
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Function
Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks. -
Sequence similarities
Belongs to the SLX4 family.
Contains 1 BTB (POZ) domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Localizes to sites of DNA dammage. - Information by UniProt
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Database links
- Entrez Gene: 84464 Human
- Omim: 613278 Human
- SwissProt: Q8IY92 Human
- Unigene: 143681 Human
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Alternative names
- BTB (POZ) domain containing 12 antibody
- BTB/POZ domain-containing protein 12 antibody
- BTBD12 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab169114 has been referenced in 2 publications.
- Fu S et al. HIV-1 exploits the Fanconi anemia pathway for viral DNA integration. Cell Rep 39:110840 (2022). PubMed: 35613597
- Park SH et al. ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress. Nat Commun 10:5718 (2019). PubMed: 31844045