In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
Our Abpromise guarantee covers the use of ab10135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 22-23 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human SM22 alpha peptide (ab23184).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 17099777
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50. PubMed: 16080196|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 26183159|
|ICC/IF||Use a concentration of 5 µg/ml.|
ab10135 staining (0.2