Overview

  • Product name
  • Description
    Rabbit polyclonal to SM22 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-188 of Human SM22 alpha (Uniprot ID: Q01995)

  • Positive control
    • HepG2 whole cell lysate, Mouse liver whole cell lysate, A-10 cells treated with MG132. HeLa cells. Ca922 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab155272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-SM22 alpha antibody (ab155272) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Anti-SM22 alpha antibody (ab155272) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • All lanes : Anti-SM22 alpha antibody (ab155272) at 1/5000 dilution

    Lane 1 : A-10 whole cell lysate
    Lane 2 : A-10 whole cell lysate treated with MG132

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 23 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling SM22 alpha with ab155272 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, labeling SM22 alpha with ab155272 at 1/100 dilution.

References

This product has been referenced in:
  • Lai YL  et al. Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells. Redox Biol 15:51-61 (2018). WB . Read more (PubMed: 29216542) »
  • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (aortic smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
aortic smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Mc Shen

Verified customer

Submitted May 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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