Overview

  • Product nameAnti-SM22 alpha antibody
    See all SM22 alpha primary antibodies
  • Description
    Mouse monoclonal to SM22 alpha
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human SM22 alpha protein (AAH04927) with tag.

  • Positive control
    • SM22 alpha transfected 293T cell lysate. Recombinant tagged Human SM22 alpha protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab77442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionActin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • Sequence similaritiesBelongs to the calponin family.
    Contains 1 calponin-like repeat.
    Contains 1 CH (calponin-homology) domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 22 kDa actin binding protein antibody
    • 22 kDa actin-binding protein antibody
    • Human 22kDa smooth muscle protein antibody
    • Protein WS3-10 antibody
    • SM22 alpha antibody
    • SM22 antibody
    • SM22-alpha antibody
    • SMCC antibody
    • Smooth muscle protein 22-alpha antibody
    • TAGL_HUMAN antibody
    • TAGLN antibody
    • TAGLN1 antibody
    • Transgelin antibody
    • Transgelin variant 2 antibody
    • WS3 10 antibody
    see all

Anti-SM22 alpha antibody images

  • All lanes : Anti-SM22 alpha antibody (ab77442) at 1 µg/ml

    Lane 1 : SM22 alpha transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • Anti-SM22 alpha antibody (ab77442) at 1 µg/ml + Recombinant tagged Human SM22 alpha protein at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 23 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-SM22 alpha antibody (ab77442)

ab77442 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"