![Western blot - Smac / Diablo antibody [8H5AB2] (ab110291) image](#Smac-Diablo-Primary-antibodies-ab110291-2.gif)
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Product name
Anti-Smac / Diablo antibody [8H5AB2]
See all Smac / Diablo products (14) ...
Description
Mouse monoclonal [8H5AB2] to Smac / Diablo
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant Smac, TIMM22 proteins
Positive control
Human liver mitochondria and HepG2 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
8H5AB2
Isotype
IgG2
Light chain type
kappa
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Mitochondrial
Cell Biology >> Apoptosis >> Receptors >> Death Receptors
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Western blot - Smac / Diablo antibody [8H5AB2] (ab110291)
![Western blot - Smac / Diablo antibody [8H5AB2] (ab110291) image](/ps/datasheet/images/110/ab110291/Smac-Diablo-Primary-antibodies-ab110291-2.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab110291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
Target
Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
Domain
The mature N-terminus mediates interaction with XIAP.
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis.
Target information above from: UniProt accessionQ9NR28
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
see all
Database links
- Entrez Gene: 56616 Human
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- Unigene: 169611 Human
Anti-Smac / Diablo antibody [8H5AB2] images:
Western blot - Smac / Diablo antibody [8H5AB2] (ab110291)
All lanes : Anti-Smac / Diablo antibody [8H5AB2] (ab110291) at 1 µg/ml
Lane 1 : Human liver mitichondria
Lane 2 : HepG2 cells
Predicted band size : 27 kDa
References for Anti-Smac / Diablo antibody [8H5AB2] (ab110291)
ab110291 has not yet been referenced specifically in any publications.
Publishing research using ab110291? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"