The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at an assay dependent dilution. Citrate buffer antigen retrieval was used.
WB: Use at a concentration of 0.5 - 2 mg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27.1 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
DomainThe mature N-terminus mediates interaction with XIAP.
Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.
Second mitochondria derived activator of caspase antibody
Second mitochondria-derived activator of caspase antibody
SMAC 3 antibody
Smac protein antibody
Anti-Smac / Diablo antibody images
Western blot - Smac / Diablo antibody (ab13817)
Predicted band size : 27.1 kDa
References for Anti-Smac / Diablo antibody (ab13817)
This product has been referenced in:
Lopergolo A et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer100:739-46 (2009).
Read more (PubMed: 19223905) »