Overview

  • Product name
    Anti-Smac / Diablo antibody
    See all Smac / Diablo primary antibodies
  • Description
    Rabbit polyclonal to Smac / Diablo
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac/DIABLO

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: Use at an assay dependent dilution. Citrate buffer antigen retrieval was used.
    WB: Use at a concentration of 0.5 - 2 mg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27.1 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
    • Tissue specificity
      Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
    • Domain
      The mature N-terminus mediates interaction with XIAP.
    • Cellular localization
      Mitochondrion. Released into the cytosol when cells undergo apoptosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0610041G12Rik antibody
      • DBLOH_HUMAN antibody
      • DBOH antibody
      • DFNA64 antibody
      • diablo antibody
      • Diablo homolog (Drosophila) antibody
      • Diablo homolog antibody
      • Diablo homolog mitochondrial antibody
      • Diablo IAP binding mitochondrial protein antibody
      • Diablo like protein antibody
      • DIABLO S antibody
      • Direct IAP binding protein with low pI antibody
      • Direct IAP-binding protein with low pI antibody
      • FLJ10537 antibody
      • FLJ25049 antibody
      • mitochondrial antibody
      • Mitochondrial Smac protein antibody
      • Second mitochondria derived activator of caspase antibody
      • Second mitochondria-derived activator of caspase antibody
      • SMAC 3 antibody
      • Smac antibody
      • Smac protein antibody
      • SMAC3 antibody
      see all

    Images



    • Predicted band size : 27.1 kDa

    References

    This product has been referenced in:
    • Lopergolo A  et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB ; Human . Read more (PubMed: 19223905) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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