Overview

  • Product nameAnti-Smac / Diablo antibody
    See all Smac / Diablo primary antibodies
  • Description
    Rabbit polyclonal to Smac / Diablo
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: LQVEEVHQLS RKAETKLAEA QIEELRQKTQ EEGEERAESE QEAYLRED, corresponding to C terminal amino acids 139-186 of Human Smac/ Diablo

  • Positive control
    • HepG2 cell lysate for WB. Paraffin embedded human kidney tissue for IHC.

Properties

Applications

Our Abpromise guarantee covers the use of ab23450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/15000.
WB Use a concentration of 1 - 1.25 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • DomainThe mature N-terminus mediates interaction with XIAP.
  • Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody images

  • Anti-Smac / Diablo antibody (ab23450) at 1 µg/ml + HepG2 lysate

    Secondary
    HRP conjugated anti-Rabbit IgG (1: 50,000-100,000)

    Predicted band size : 27 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • ab23450 at 4-8 ug/ml staining human Smac in kidney cells by immunohistochemistry (Paraffin embedded tissue). Epithelial cells of renal tubule (indicated with arrows). Magnification 400x.

References for Anti-Smac / Diablo antibody (ab23450)

ab23450 has not yet been referenced specifically in any publications.

Product Wall

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