Overview

  • Product nameAnti-Smac / Diablo antibody
    See all Smac / Diablo primary antibodies
  • Description
    Mouse monoclonal to Smac / Diablo
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Cynomolgus Monkey, Rhesus monkey, Orangutan
  • Immunogen

    Recombinant tagged fragment: SLLGKMNSEE EDEVWQVIIG ARAEMTSKHQ EYLKLETTWM TAVGLSEMAA EAAYQTGADQ ASITARNHIQ LVKLQVEEVH QLSRKAETKL , corresponding to amino acids 119-218 of Human Smac/Diablo.

  • Positive control
    • Smac/Diablo transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Smac/Diablo is approximately 0.3ng/ml when used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • DomainThe mature N-terminus mediates interaction with XIAP.
  • Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody images

  • All lanes : Anti-Smac / Diablo antibody (ab68352) at 1 µg/ml

    Lane 1 : Smac / Diablo transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
  • ab68352 at 1µg/well as a capture antibody in Sandwich ELISA. Detection limit for recombinant tagged Smac/Diablo is approximately 0.3ng/ml as a capture antibody.

References for Anti-Smac / Diablo antibody (ab68352)

ab68352 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"