The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged Smac/Diablo is approximately 0.3ng/ml when used as a capture antibody.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
DomainThe mature N-terminus mediates interaction with XIAP.
Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.