Overview

  • Product nameAnti-Smac / Diablo antibody
    See all Smac / Diablo primary antibodies
  • Description
    Rabbit polyclonal to Smac / Diablo
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide (

    EERAESEQEAYLRED

    )corresponding to amino acids 225 to 239 of humanSmac/DIABLO (1).

  • Positive control
    • Human heart tissue lysate. IF/ICC: MCF7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab8114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.Can be blocked with Smac / Diablo peptide (ab8380).

Target

  • FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • DomainThe mature N-terminus mediates interaction with XIAP.
  • Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody images

  • All lanes : Anti-Smac / Diablo antibody (ab8114) at 1 µg/ml

    Lane 1 : human heart tissue lysate with absence of blocking peptide
    Lane 2 : human heart tissue lysate with Smac / Diablo peptide (ab8380)


    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab8114 at 5µg/ml staining Smac/Diablo in human ovary tissue by IHC

  • ICC/IF image of ab8114 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Smac / Diablo antibody (ab8114)

This product has been referenced in:
  • Bannwarth S  et al. A mitochondrial origin for frontotemporal dementia and amyotrophic lateral sclerosis through CHCHD10 involvement. Brain 137:2329-45 (2014). Read more (PubMed: 24934289) »

See 1 Publication for this product

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Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully unde...

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Same answer again I am afraid! To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"