Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)

Overview

  • Product nameAnti-Smac / Diablo antibody [SMAC 17 1-87]
    See all Smac / Diablo primary antibodies
  • Description
    Mouse monoclonal [SMAC 17 1-87] to Smac / Diablo
  • Tested applicationsSuitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length Human Smac/ Diablo with a His tag at C terminal end.

  • Positive control
    • Human tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab111893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF 1/20 - 1/200.
WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).

Target

  • FunctionPromotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
  • Tissue specificityUbiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
  • DomainThe mature N-terminus mediates interaction with XIAP.
  • Cellular localizationMitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610041G12Rik antibody
    • DBLOH_HUMAN antibody
    • DBOH antibody
    • DFNA64 antibody
    • diablo antibody
    • Diablo homolog (Drosophila) antibody
    • Diablo homolog antibody
    • Diablo homolog mitochondrial antibody
    • Diablo IAP binding mitochondrial protein antibody
    • Diablo like protein antibody
    • DIABLO S antibody
    • Direct IAP binding protein with low pI antibody
    • Direct IAP-binding protein with low pI antibody
    • FLJ10537 antibody
    • FLJ25049 antibody
    • mitochondrial antibody
    • Mitochondrial Smac protein antibody
    • Second mitochondria derived activator of caspase antibody
    • Second mitochondria-derived activator of caspase antibody
    • SMAC 3 antibody
    • Smac antibody
    • Smac protein antibody
    • SMAC3 antibody
    see all

Anti-Smac / Diablo antibody [SMAC 17 1-87] images

  • ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab111893 labelling Smac / Diablo (green) in the cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab111893 labelling Smac / Diablo (green) in the cytoplasm of PC12 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • All lanes : Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 27 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

References for Anti-Smac / Diablo antibody [SMAC 17 1-87] (ab111893)

ab111893 has not yet been referenced specifically in any publications.

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