RabMAb

Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)

Overview

  • Product nameAnti-SMAD 1 + SMAD9 antibody [EPR2290(2)]
  • Description
    Rabbit monoclonal [EPR2290(2)] to SMAD 1 + SMAD9
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SMAD 1 + SMAD9.

  • Positive control
    • 293T, A673, Human heart and HeLa lysates
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
    • Sequence similaritiesBelongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • MAD homolog 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] images

    • All lanes : Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : A673 lysate
      Lane 3 : Human heart lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1, 3 & 4 : HRP labelled goat anti-rabbit at 1/2000 dilution


      Predicted band size : 52 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)

    References for Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)

    ab108965 has not yet been referenced specifically in any publications.

    Product Wall

    We carry products which recognize mouse SMAD1/9, SMAD2/3 or SMAD1/5/9. There is no antibody currently on our catalog which recognizes all forms of SMAD in mouse. I have added links to our anti- SMAD1/9, SMAD2/3 and SMAD1/5/9 antibodies for your review ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"