Anti-Smad Interacting Protein 1 antibody (ab113655)

Overview

  • Product name
    Anti-Smad Interacting Protein 1 antibody
    See all Smad Interacting Protein 1 primary antibodies
  • Description
    Goat polyclonal to Smad Interacting Protein 1
  • Specificity
    This antibody is expected to recognize both reported isoforms (NP_055610.1; NP_001165124.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Xenopus laevis, Zebrafish, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    TDSRRQISNIKKEK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 545-558 of Human Smad Interacting Protein 1 (according to NP_055610.1; NP_001165124.1).

  • Positive control
    • Human Liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 38, 100 kDa (predicted molecular weight: 136 kDa). Primary incubation time: 1 hour.
ELISA 1/32000.

Target

  • Function
    Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
  • Involvement in disease
    Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS) [MIM:235730]; also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes.
  • Sequence similarities
    Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation on Lys-391 and Lys-866 is promoted by the E3 SUMO-protein ligase CBX4, and impairs interaction with CTBP1 and transcription repression activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42816 antibody
    • HSPC082 antibody
    • KIAA0569 antibody
    • SIP 1 antibody
    • SIP1 antibody
    • Smad-interacting protein 1 antibody
    • SMADIP 1 antibody
    • SMADIP1 antibody
    • ZEB 2 antibody
    • Zeb2 antibody
    • ZEB2_HUMAN antibody
    • Zfhx1b antibody
    • ZFHX1B protein antibody
    • Zfx1b antibody
    • Zinc finger E box binding protein 2 antibody
    • Zinc finger E-box-binding homeobox 2 antibody
    • Zinc finger homeobox 1b antibody
    • zinc finger homeobox protein 1 antibody
    • Zinc finger homeobox protein 1b antibody
    see all

Images

  • Anti-Smad Interacting Protein 1 antibody (ab113655) at 0.2 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg

    Predicted band size : 136 kDa
    Observed band size : 38,100 kDa (why is the actual band size different from the predicted?)

References

ab113655 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (gel 8%)
Sample
Human Cell lysate - whole cell (ovarian cancer cell line SKOV3)
Specification
ovarian cancer cell line SKOV3
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
Username

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Verified customer

Submitted Aug 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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