Recombinant
RabMAb

Anti-Smad1 antibody [EPR5522] (ab126761)

Overview

  • Product name
    Anti-Smad1 antibody [EPR5522]
    See all Smad1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5522] to Smad1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Smad1.

  • Positive control
    • HeLa, A673, HepG2, and 293T cell lysate; Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Tissue specificity
      Ubiquitous. Highest expression seen in the heart and skeletal muscle.
    • Sequence similarities
      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications
      Phosphorylated on serine by BMP type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BSP-1 antibody
      • BSP1 antibody
      • HsMAD1 antibody
      • JV4-1 antibody
      • JV41 antibody
      • MAD homolog 1 antibody
      • MAD mothers against decapentaplegic homolog 1 antibody
      • Mad related protein 1 antibody
      • Mad-related protein 1 antibody
      • MADH1 antibody
      • MADR1 antibody
      • Mothers against decapentaplegic homolog 1 antibody
      • Mothers against DPP homolog 1 antibody
      • SMA- AND MAD-RELATED PROTEIN 1 antibody
      • SMAD 1 antibody
      • SMAD family member 1 antibody
      • SMAD mothers against DPP homolog 1 antibody
      • Smad1 antibody
      • SMAD1_HUMAN antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta-signaling protein 1 antibody
      see all

    Images

    • All lanes : Anti-Smad1 antibody [EPR5522] (ab126761) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 52 kDa
    • ab126761, at 1/50 dilution, staining Smad1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126761 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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