Anti-Smad1 (phospho S206) antibody (ab106092)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S206)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smad1 (phospho S206) antibody
See all Smad1 primary antibodies -
Description
Rabbit polyclonal to Smad1 (phospho S206) -
Host species
Rabbit -
Specificity
ab106092 is specific for Smad1 phosphorylated pS206. Reactivity with nonphosphorylated human Smad1 is minimal by ELISA and western blot. Espected to recognize Smad1 from Duckbill platypus, bonobo, salmon due to 100% immunogen seqeunce homology. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Chicken, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Macaque monkey, Gorilla, Opossum, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Smad1 (phospho S206).
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Positive control
- HEPG2 and HaCaT whole cell lysates, treated with BMP2, EGF, or NaCl.
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General notes
Expiration date is one year from date of opening. Dilute only prior to immediate use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106092 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500.
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ELISA |
Use at an assay dependent concentration.
|
Notes |
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WB
1/500. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Tissue specificity
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 540488 Cow
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Omim: 601595 Human
- SwissProt: Q1JQA2 Cow
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- Unigene: 604588 Human
see all -
Alternative names
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
Datasheets and documents
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Datasheet download
References (0)
ab106092 has not yet been referenced specifically in any publications.