Anti-Smad1 (phospho S206) antibody (ab106093)

Overview

  • Product nameAnti-Smad1 (phospho S206) antibody
    See all Smad1 primary antibodies
  • Description
    Rabbit polyclonal to Smad1 (phospho S206)
  • Specificityab106093 is specific for Human Smad1 phosphorylated at pS206. Reactivity with nonphosphorylated Human Smad1 is minimal by ELISA and western blot.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Macaque Monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    A phosphorylated synthetic peptide corresponding to amino acids surrounding serine 206 of Human Smad1.

  • Positive control
    • HEPG2 whole cell lysates treated with BMP2, EGF, or NaCl.
  • General notesExpiration date is one year from date of opening.

Properties

Applications

Our Abpromise guarantee covers the use of ab106093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/5000 - 1/25000.

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Anti-Smad1 (phospho S206) antibody images

  • All lanes : Anti-Smad1 (phospho S206) antibody (ab106093) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate, treated with PBS
    Lane 2 : HepG2 whole cell lysate, treated with BMP2 (5 ng/ml)
    Lane 3 : HepG2 whole cell lysate, treated with BMP2 (5 ng/ml)
    Lane 4 : HepG2 whole cell lysate, treated with NaCL

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated a-Rabbit IgG at 1/3000 dilution

    Predicted band size : 52 kDa

References for Anti-Smad1 (phospho S206) antibody (ab106093)

ab106093 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"