ab1060093 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.
Phosphorylated on serine by BMP type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
MAD mothers against decapentaplegic homolog 1 antibody
Mad related protein 1 antibody
Mad-related protein 1 antibody
Mothers against decapentaplegic homolog 1 antibody
Mothers against DPP homolog 1 antibody
SMA- AND MAD-RELATED PROTEIN 1 antibody
SMAD 1 antibody
SMAD family member 1 antibody
SMAD mothers against DPP homolog 1 antibody
TGF beta signaling protein 1 antibody
Transforming growth factor-beta-signaling protein 1 antibody
Western blot - Anti-Smad1 (phospho S206) antibody (ab106093)
All lanes : Anti-Smad1 (phospho S206) antibody (ab106093) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate, treated with PBS Lanes 2-3 : HepG2 whole cell lysate, treated with BMP2 (5 ng/ml) Lane 4 : HepG2 whole cell lysate, treated with NaCL
Lysates/proteins at 15 µg per lane.
Secondary All lanes : HRP-conjugated a-Rabbit IgG at 1/3000 dilution
Predicted band size: 52 kDa
Cell lysates were incubated for 1 h at 37°C.
Primary antibody was pre-incubated before reacting with blot as follows: top row - with PBS, middle row - with the immunizing phosphorylated peptide bottom row - with control or non-phosphorylated peptide.