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PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam's PhosphoTracer SMAD1 (pSer463/465) assay kits detect endogenous levels of SMAD1 (GenBank Accession NP_001003688) in cellular lysates. Phospho-SMAD1 assay kits only detect SMAD1 when phosphorylated at Ser463/465. Total SMAD1 assay kits detect SMAD1 irrespective of phosphorylation status. Based on sequence similarity, cross reaction to SMAD5 and SMAD8 may occur.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution).
Learn more about the fluorogenic substrate, ADHP.
|Components||1 x 96 tests|
|96-well PhosphoTracer assay plate (stripwell)||1 unit|
|Adherent plate seal||2 units|
|ADHP (100X)||1 x 120µl|
|ADHP Dilution Buffer||1 x 15ml|
|Assay Control Lysate (lyophilized)||1 x 0.25ml|
|Enhancer Solution||1 x 1ml|
|Lysis Buffer (5X)||1 x 15ml|
|Mouse monoclonal SMAD1 (HRP) (96 assay points)||1 x 3ml|
|Phospho SMAD1 (Ser463/465) (96 assay points)||1 x 3ml|
|Stop Solution||1 x 2ml|
|Wash Buffer (10X)||1 x 15ml|
Our Abpromise guarantee covers the use of ab119647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Tested in HeLa, C2C12.|
ab119647 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"